• 基础环境为 jupyter/r-notebook

CellChat

conda create -n mamba conda-forge::mamba
source activate mamba
mamba create -n cellchat conda-forge::r-irkernel conda-forge::r-tidyverse conda-forge::r-devtools conda-forge::r-circlize
conda run -n cellchat Rscript -e "IRkernel::installspec(name='r-cellchat', displayname='r-cellchat')"
mamba install -n cellchat bioconda::bioconductor-biobase \
bioconda::bioconductor-complexheatmap bioconda::bioconductor-biocneighbors
mamba install -n cellchat conda-forge::r-ggpubr  conda-forge::r-ggnetwork conda-forge::r-rstatix \
conda-forge::r-car conda-forge::r-pbkrtest conda-forge::r-lme4 \
conda-forge::r-igraph conda-forge::r-nloptr conda-forge::r-svglite

cd /tmp/
wget https://github.com/jinworks/CellChat/archive/refs/heads/main.zip -O CellChat-master.zip
unzip CellChat-master.zip
cd CellChat-main/src/
rm *.so *.o
cd /tmp/

source activate cellchat
proxychains R

install.packages('NMF')
devtools::install_local('CellChat-main')

packageVersion("CellChat")